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Antisense Display: A New Method for Functional Gene Screen and Its Application to Angiogenesis‐Related Gene Isolation
Author(s) -
YONEKURA HIDETO,
YASUI KIYOSHI,
SAKURAI SHIGERU,
LI HUI,
YAMAMOTO YASUHIKO,
YAMAMOTO HIROSHI
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03968.x
Subject(s) - gene , angiogenesis , biology , phenotype , rab , gene expression , transfection , microbiology and biotechnology , computational biology , genetics , gtpase
A bstract : We have developed an antisense‐oriented method for functional gene screening, which we termed “antisense display.” After evaluation in a cell‐free system, we applied this method to a cellular system for isolating angiogenesis‐related genes and came across a gene previously not described in humans. A screen of 10 mer antisense repertoire led to the identification of a unique ‘angiostatic’ antisense, and the gene corresponding to it was found to be preferentially expressed in vascular endothelial cells, coding for a putative type‐2 membrane protein containing Rab‐GAP/TBC and EF‐hand‐like domains. We named it vascular Rab‐GAP/TBC domain‐containing protein (VRP). The results obtained thus indicate that the antisense display method is useful for the isolation of functional genes responsible for phenotypes concerned.