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Lysophosphatidylcholine Inhibits T Cell‐Specific CXC Chemokines IP‐10, MIG, and I‐TAC Expression Induced by IFN‐γ in Human Endothelial Cells
Author(s) -
SHEIKH ABDULLAH MD.,
OCHI HIROSHI,
MASUDA JUNICHI
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03952.x
Subject(s) - lysophosphatidylcholine , chemokine , monokine , chemistry , cxc chemokine receptors , microbiology and biotechnology , immune system , interferon gamma , cytokine , biology , immunology , chemokine receptor , biochemistry , phospholipid , membrane , phosphatidylcholine
A bstract : Hypercholesterolemia is reported to be associated with a T helper (Th) 1 to Th2 switch in apoE‐deficient mice. To explore the underlying mechanisms of such immune modulation, we investigated if lysophosphatidylcholine (Lyso‐PC), enriched in oxidized LDL, affects cytokine‐induced expression of Th1 cell‐specific CXC chemokines, IFN‐inducible protein of 10 kDa (IP‐10), monokine induced by IFN‐γ (Mig), and IFN‐inducible T cell a chemoattractant (I‐TAC) in cultured endothelial cells. Lyso‐PC inhibited IFN‐γ‐ or IFN‐γ plus TNF‐α‐induced IP‐10, Mig, and I‐TAC mRNA expression but not that of the IRF‐1 or IRF‐1‐dependent molecule, GBP. It also inhibited IP‐10 protein secretion in conditioned media induced by IFN‐γ or IFN‐γ plus TNF‐α. Western analysis demonstrated that Lyso‐PC inhibited IFN‐γ‐induced STAT‐1α protein expression, but not that of IRF‐1. These results suggest that Lyso‐PC selectively inhibits IP‐10, Mig, and I‐TAC expression through IRF‐1‐independent mechanisms. This inhibitory effect of Lyso‐PC may be involved in the immune modulation by hypercholesterolemia.