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Low‐Molecular‐Weight DNA in Blood Plasma as an Index of the Influence of Ionizing Radiation
Author(s) -
VASILYEVA IRI.
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03889.x
Subject(s) - dna , nucleosome , ionizing radiation , chemistry , genomic dna , irradiation , microbiology and biotechnology , histone , biology , biochemistry , physics , nuclear physics
A bstract : Low‐molecular‐weight DNA (180 bp) appears in blood plasma of irradiated rats. Its amount correlates directly with the irradiation dose. The cloned low‐molecular‐weight DNA is enriched with G/C pairs (48% compared with 40% in the rodent genome). Computer search against EMBL data showed that the sequence of these clones is homologous to various genomic repetitive elements. The program for identification of nucleosome‐positioning sequences in eukaryotic nuclear DNA has shown that low‐molecular‐weight DNA fragments are able to form rather sustainable nucleosomes and have some peculiarity. The difference in the composition of low‐molecular‐weight DNA isolated from blood plasma of rats after irradiation at doses of 8 and 100 Gy consisted of the change in the levels of two dinucleotides (CpG and CpT). The DNA in question originates from different types of cells.