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Detection of Maternal DNA in Umbilical Cord Plasma by Fluorescent PCR Amplification of Short Tandem Repeat Sequences
Author(s) -
BAUER MARGIT,
ORESCOVIC IRMGARD,
SCHOELL WOLFGANG M.,
BIANCHI DIANA W.,
PERTL BARBARA
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03880.x
Subject(s) - umbilical cord , minisatellite , polymerase chain reaction , biology , cord blood , dna , microbiology and biotechnology , microsatellite , tandem repeat , genetics , gene , genome , allele
A bstract : Recently, maternal DNA was detected in umbilical cord blood using PCR amplification of minisatellite sequences. The presence of maternal DNA was demonstrated in 1% to 100% of umbilical cord blood samples. The objective of this study was to determine the frequency of cord blood contamination by maternal genetic material. We used fluorescent PCR amplification of highly polymorphic short tandem repeat (STR) markers to detect maternal DNA in umbilical cord plasma.