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Technical Optimization of RhD Zygosity Determination by Real‐Time Quantitative Polymerase Chain Reaction
Author(s) -
CHIU ROSSA W. K.,
MURPHY MICHAEL F.,
FIDLER CARRIE,
WAINSCOAT JAMES S.,
LO Y. M. DENNIS
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03879.x
Subject(s) - zygosity , multiplex , real time polymerase chain reaction , polymerase chain reaction , multiplex polymerase chain reaction , computational biology , microbiology and biotechnology , chemistry , chromatography , biology , gene , genetics , biochemistry
A bstract : We have recently reported the development of a multiplex, real‐time quantitative polymerase chain reaction (PCR) assay for RhD zygosity determination based on the coamplification and quantification of RHD ‐specific sequences in relation to a reference gene, albumin . This paper discusses the optimization procedure and technical parameters of this multiplex assay.