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Fetal DNA Diagnosis from Maternal Blood
Author(s) -
IKAWA K.,
YAMAFUJI K.,
UKITA T.,
KUWABARA S.,
IGARASHI T.,
TAKABAYASHI H.
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03878.x
Subject(s) - taqman , dna , locus (genetics) , real time polymerase chain reaction , fetus , microbiology and biotechnology , biology , gene , genetics , pregnancy
A bstract : Development of a real‐time quantitative TaqMan PCR assay permits the high‐quality analysis of DNA or RNA. This study demonstrates the successful detection of single locus genes from single NRBCs, retrieved by micromanipulation of cells on a blood smear slide. Quantitative data in relation to the concentration of a single locus DNA amplified by PEP from a single NRBC are also provided. In summary, a PEP‐TaqMan system can potentially be a powerful tool for noninvasive fetal DNA diagnosis through the use of maternal blood.