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An Approach to Constructing Three‐Dimensional Tissue
Author(s) -
KO IN KAP,
IWATA HIROO
Publication year - 2001
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2001.tb03854.x
Subject(s) - cellulase , cellulose , fibronectin , tissue engineering , fiber , chemistry , biophysics , cellulose fiber , digestion (alchemy) , cell , biomedical engineering , chromatography , biochemistry , biology , organic chemistry , medicine
A bstract : The authors propose an approach to constructing three‐dimensional tissue with capillaries using cellulose hollow fibers. Fibronectin (FN) was immobilized on hollow fibers to assure cell attachment. Bovine coronary artery smooth muscle cells (BCASMC) and L cells were seeded on FN‐immobilized fibers and cultured for an extended period of time. The cells proliferated and formed multicellular layers on the fibers. The hollow fibers were removed by enzymatic digestion using cellulase. The cellulase treatment did not damage L cells, although some cells fell off from the fibers. On the other hand, no deterioration was observed in the BCASMC aggregate structure. The BCASMC aggregates maintained several lumens after removal of the hollow fibers by cellulase digestion. The authors believe that their approach offers a useful method to tissue engineering in preparation of three‐dimensional tissue structure.