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Nerve Growth Factor Upregulates the PAC 1 Promoter by Activating the MAP Kinase Pathway in Rat PC12 Cells
Author(s) -
JAMEN F.,
LADEN J. C.,
BOUSCHET T.,
RODRIGUEZHENCHE N.,
BOCKAERT J.,
BRABET P.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb07002.x
Subject(s) - nerve growth factor , promoter activity , transcription factor , microbiology and biotechnology , neurotrophin , chemistry , kinase , cell growth , mitogen activated protein kinase , signal transduction , biology , promoter , receptor , gene expression , biochemistry , gene
We report that: (1) An increase in the transcription activity is a mechanism by which trophic peptides may regulate the expression of PAC1. (2) An activation of the PAC1 promoter does not necessarily correlate with the neurotrophin-promoted neuritogenesis. (3) Activation of the PAC1 promoter is probably an early event since the EGF response is rather weak and transient in PC12 cells. (4) MAP kinase pathway activation is necessary for the NGF effect. The mechanism of the antagonism between PACAP and NGF observed on the PAC1 promoter activity and already described in regulating chromaffin cell proliferation, remains to be explained.