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PACAP Peptides Modulate Guinea Pig Cardiac Neuron Membrane Excitability and Neuropeptide Expression
Author(s) -
PARSONS RODNEY L.,
ROSSIGNOL TODD M.,
CALUPCA MICHELLE A.,
HARDWICK JEAN C.,
BRAAS KAREN M.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06967.x
Subject(s) - medicine , endocrinology , neuropeptide , cholinergic , neuropeptide y receptor , neuron , somatostatin , biology , receptor , chemistry , microbiology and biotechnology , neuroscience
A bstract : Morphological studies identified PACAP‐immunoreactive nerve fibers in dense pericellular arrangements around virtually every cholinergic parasympathetic neuron of guinea pig cardiac ganglia; all postganglionic cardiac neurons expressed membrane‐associated PAC 1 receptor protein. Characterization of the alternative splice variants established predominant expression of the PAC 1 ( very short ) receptor transcript containing neither HIP nor HOP exons. PACAP depolarized cardiac neurons and increased membrane excitability; the excitability resulted from neither altered action potential properties nor inhibition of I M . Treatment of cardiac ganglia explants with PACAP significantly reduced the numbers of cholinergic neurons coexpressing somatostatin immunoreactivity, which did not appear to be correlated with prosomatostatin mRNA expression. The PACAP‐mediated decrease in somatostatin immunoreactive neurons required calcium influx through L‐type calcium channels and activation of adenylyl cyclase, whereas activation of phospholipase C or protein kinase A was not required. These observations indicate that PACAP through the PAC 1 receptors elicits complex actions on guinea pig parasympathetic cardiac ganglia neurons, including modulation of membrane ion conductances and modulation of neuropeptide expression.