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Loss of Heterozygosity at the ACTBP2 Locus in Lung Cancer Detected on Elchrom Precast Spreadex Gels
Author(s) -
KOZULIC MIRJANA,
CHEN XU QI,
BONILLA FELIX,
SILVA JOSE
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06595.x
Subject(s) - loss of heterozygosity , microsatellite , locus (genetics) , allele , microbiology and biotechnology , microsatellite instability , polyacrylamide gel electrophoresis , precast concrete , lung cancer , biology , chemistry , genetics , pathology , medicine , biochemistry , gene , history , archaeology , enzyme
A bstract : One of the most important goals in the continuous struggle against cancer is early detection of the disease by a simple method that can be used in routine laboratory practice. According to several recent reports, early detection may be achieved by monitoring alterations at several microsatellite loci containing tri‐ and tetranucleotide repeats. The method relies on comparison of the intensity of allele bands in cancer tissue, plasma, and lymphocytes of a patient. Microsatellite instability is typically detected by labeling the DNA with a radioactive probe or a fluorescent dye followed by electrophoresis on 20–40‐cm‐long denaturing polyacrylamide gels. In this study, we show that loss of heterozygosity at the ACTBP2 locus in lung cancer patients can be detected using the Elchrom electrophoresis system and just 4‐cm‐long precast Spreadex gels.