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Group IV Cytosolic Phospholipase A 2 Activation by Diacylglycerol Pyrophosphate in Murine P388D 1 Macrophages
Author(s) -
BALSINDE JESÚS,
BALBOA MARÍA A.,
DENNIS EDWARD A.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb06533.x
Subject(s) - diacylglycerol kinase , phospholipase a2 , phosphatidate , cytosol , arachidonic acid , phospholipid , biochemistry , phospholipase , pyrophosphate , phospholipase a , phospholipase d , chemistry , lipid signaling , microbiology and biotechnology , biology , enzyme , protein kinase c , membrane
A bstract : Diacylglycerol pyrophosphate (DGPP) is a novel phospholipid identified in yeast, bacteria, and plants, but not yet in mammalian cells. Given its structural resemblance to other phospholipid‐activating molecules, such as lysophosphatidate, phosphatidate, and diacylglycerol, it was questioned whether DGPP was capable of activating macrophages to release arachidonic acid (AA) metabolites such as the prostaglandins. It has been found that DGPP is able to potently stimulate prostaglandin production in the murine cell line P388D 1 by a mechanism that involves activation of the cytosolic Group IV phospholipase A 2 (cPLA 2 ). Our results demonstrate that DGPP possesses macrophage‐activating‐factor properties and suggest a role for this novel compound in the inflammatory response.