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Proliferation and Cytokine Profile of T. annulata ‐Infected Ovine, Caprine, and Bovine Lymphoblastoid Cells
Author(s) -
SCHNITTGER L.,
HOLLMANN C.,
DIEMER U.,
BOGUSLAWSKI K.,
AHMED J. S.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05360.x
Subject(s) - biology , cytokine , messenger rna , flow cytometry , microbiology and biotechnology , in vitro , cell culture , parasite hosting , lymphoblast , in vivo , virology , immunology , gene , genetics , world wide web , computer science
A bstract :T. annulata , the causative agent of tropical theileriosis in cattle, can also infect ovine and caprine leukocytes in vitro . In vivo studies showed that this parasite causes a mild infection in both these animal species, and in sheep merozoite stage development seems to be inhibited. Since the nature of T. annulata infected caprine and ovine cells is not known, all three cell lines were karyotyped and phenotypically characterized by flow cytometry. They all express mRNA of cytokines IL‐1α, IL‐1β, IL‐8, and TNF‐α, but not of IFN‐γ, IL‐2, and IL‐4. In contrast, IL‐6 mRNA was expressed in the cattle cell line only, while mRNA of IL‐10 was exclusively produced by the sheep cell line. The observed differences in cytokine mRNA expression may be responsible for the different pathogenesis of T. annulata infection in cattle and sheep.