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Integrated Molecular Diagnosis of Theileria and Babesia Species of Cattle in Italy
Author(s) -
SPARAGANO O.,
LORIA G. R.,
GUBBELS M.J.,
VOS A. P.,
CARACAPPA S.,
JONGEJAN F.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05332.x
Subject(s) - babesia bovis , babesia bigemina , babesia , biology , theileria , virology , veterinary medicine , ovis , anaplasma , trypanosoma vivax , babesiosis , trypanosoma , parasite hosting , tick , medicine , ecology , world wide web , computer science
A bstract : A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. ( T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi , and T. buffeli/orientalis ) and three Babesia spp. ( B. bovis, B. bigemina , and B. divergens ). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense , and T. vivax ). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.