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Laboratory Vector Competence of Black Flies (Diptera: Simuliidæ) for the Indiana Serotype of Vesicular Stomatitis Virus
Author(s) -
MEAD DANIEL G.,
RAMBERG FRANK B.,
MARÉ C. JOHN
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05323.x
Subject(s) - vesicular stomatitis , vesicular stomatitis virus , serotype , biology , black fly , virology , virus , vector (molecular biology) , rhabdoviridae , saliva , veterinary medicine , zoology , larva , ecology , medicine , genetics , biochemistry , gene , recombinant dna
A bstract : In previous experiments we have demonstrated that colonized and wild black flies are competent laboratory vectors of different Mexican and Western USA isolates of vesicular stomatitis virus, serotype New Jersey (VSV‐NJ). We have recently demonstrated biological VSV‐NJ transmission by black flies using animal models. In the study described here, we tested the vector competence of colonized and wild black flies for the vesicular stomatitis virus, serotype Indiana (VSV‐IN). A 1998 equine isolate was used. After a 10 day incubation period, saliva from experimentally infected Simulium vittatum and S. notatum was individually collected and tested for the presence of infectious virus. Virus was detected in the saliva of both species following oral infection, indicating that they are competent laboratory vectors of VSV‐IN. In addition, the results suggest that the black fly gut may exert evolutionary pressures on the virus.