Premium
An in Vivo Model to Investigate Lymphocyte‐Mediated Immunity During Acute Hemoparasitic Infections: Use of a Monoclonal Antibody to Selectively Deplete CD4 + T Lymphocytes from Thymectomized Calves
Author(s) -
VALDEZ R. A.,
McGUIRE T. C.,
BROWN W. C.,
DAVIS W. C.,
KNOWLES D. P.
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05294.x
Subject(s) - spleen , immunology , peripheral blood mononuclear cell , monoclonal antibody , lymph , t lymphocyte , lymphocyte , lymph node , lymphatic system , immunity , antibody , medicine , biology , immune system , in vitro , pathology , biochemistry
A bstract : Thymectomized calves were selectively depleted of CD4 + T lymphocytes with a monoclonal antibody (mAb) specific for the bovine CD4 monomer (ILA‐11). Calves were treated with high loading doses of ILA‐11 during the first week of the study then treated with subsequent lower maintenance doses. Depletion of CD4 + T lymphocytes was assessed weekly by flow cytometric analysis of PBMC and mononuclear cells from lymph node and spleen biopsies. Treatment with high doses of ILA‐11 resulted in rapid and marked depletion of CD4 + T lymphocytes from the peripheral blood, peripheral lymph nodes, and spleen. Although CD4 + T lymphocytes slowly returned to the peripheral blood, peripheral lymph nodes, and spleen by day 21 post‐treatment, the numbers of CD4 + T lymphocytes in depleted calves remained below pre‐depletion levels for the duration of the study. CD4 + T lymphocytes failed to be effectively depleted from a non‐thymectomized calf treated with the mAb ILA‐11. Development of a T lymphocyte depletion model in thymectomized calves will permit testing of the hypothesis that CD4 + T lymphocytes and IFN‐γ are required in cattle for control of acute anaplasmosis. In subsequent planned studies, thymectomized calves depleted of CD4 + T lymphocytes will be experimentally infected with A. marginale and parameters of disease compared between depleted and non‐depleted calves.