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Three 5′‐Variant mRNAs of Anion Exchanger AE2 in Stomach and Intestine of Mouse, Rabbit, and Rat
Author(s) -
ROSSMANN HEIDI,
ALPER SETH L.,
NADER MANUELA,
WANG ZHUO,
GREGOR MICHAEL,
SEIDLER URSULA
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05226.x
Subject(s) - gene isoform , stomach , secretion , small intestine , messenger rna , western blot , large intestine , microbiology and biotechnology , anion exchanger , biology , sodium–hydrogen antiporter , northern blot , epithelial polarity , gene , chemistry , biochemistry , ion exchange , cell , ion , sodium , organic chemistry
A bstract : AE2 is one of three known isoforms of the anion exchanger (AE) gene family. The use of alternative promoters, resulting in a tissue‐specific transcript pattern, was reported for all AE genes. Three N‐terminal variant AE2 subtypes are described: AE2a, AE2b, and AE2c. Although the basolaterally located parietal cell anion exchanger is known to be an AE2, the molecular identity of the basolateral and apical anion exchangers throughout the gut are still unknown. This article summarizes functional, immunohistochemical, and Western blot data demonstrating the basolateral localization of the gastric and intestinal AE2 in rabbit, mouse, and rat, and showing the AE2 subtype mRNA expression pattern in the stomach and along the intestine of rabbit and mouse: AE2a is expressed in all studied tissues, but most strongly in the colon; AE2b is expressed mainly in the stomach; and AE2c is detected nearly exclusively in the stomach. Further investigation is necessary to characterize the apical anion transport protein involved in NaCl absorption and HCO 3 − secretion in the gut.