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Serum Withdrawal Potentiates the Toxic Effects of Methamphetamine in Vitro
Author(s) -
CADET JEAN LUD,
ORDONEZ SONIA
Publication year - 2000
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2000.tb05186.x
Subject(s) - meth , methamphetamine , apoptosis , programmed cell death , reactive oxygen species , chemistry , in vitro , in vivo , cytoplasm , microbiology and biotechnology , cell damage , pharmacology , biology , biochemistry , monomer , organic chemistry , acrylate , polymer
Methamphetamine (METH) has been shown to cause neurotoxic damage both in vitro and in vivo . The mechanisms of action are thought to involve the production of pathophysiologic concentration of free radicals. The present study was undertaken to assess the toxic effects of METH caused dose‐dependent increased production of reactive oxygen species (ROS) and cell death. Cell death caused by METH was characterized by cytoplasmic vacuolar formation, shrinkage of cytoplasm and nuclear dissolution. Flow cytometric evaluation also revealed that this toxin causes changes similar to those observed in cells undergoing apoptosis. When taken together these observations suggest the METH can cause these cells to die via apoptosis. Further experiments indicated that growth of these cells in low (1%) serum or in the absence of serum markedly enhanced the apoptotic effects of METH. These data provide further support for the ideas that METH can cause ROS‐mediated apoptosis.