Premium
Studies on Conditional Gene Expression in the Brain
Author(s) -
JERECIC JASNA,
SINGLE FRANK,
KRÜTH ULI,
KRESTEL HEINZ,
KOLHEKAR ROHINI,
STORCK THORSTEN,
KASK KALEV,
HIGUCHI MIYOKO,
SPRENGEL ROLF,
SEEBURG PETER H.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb11271.x
Subject(s) - gene silencing , gene , biology , intron , genetics , gene expression , allele , regulation of gene expression , postsynaptic potential , microbiology and biotechnology , computational biology , receptor
This manuscript summarizes our recent attempts to regulate in vitro and in vivo the expression of genes encoding components and regulators of the postsynaptic machinery along with marker genes such as lacZ and GFP. In particular, we studied tTA‐dependent regulation and utilized Cre in combination with reversible silencing by intron engineering of dominant negative alleles. We further present a “knockin” approach for on‐site artificial regulation of chromosomal genes.