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Imidazoline Receptor Antisera‐Selected cDNA Clone and mRNA Distribution a
Author(s) -
PILETZ JOHN E.,
JONES JULIA CAY,
ZHU HE,
BISHARA OSAMA,
ERNSBERGER PAUL
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb09335.x
Subject(s) - microbiology and biotechnology , complementary dna , imidazoline receptor , messenger rna , antiserum , biology , gene expression , rna , housekeeping gene , receptor , gene , endocrinology , biochemistry , antibody , immunology
A novel cDNA, designated Imidazoline Receptor Antisera‐Selected cDNA‐1 ( iras‐1 ), encodes a 167‐kD protein. Two of its predicted peptides (42–43 kD) are immunologically consistent with a previously reported 1 1 ‐imidazoline binding protein. In the present study, two forms of iras mRNA (6.0 and 9.5 kb) were quantified across fresh rat tissues. Highest levels were found in brain (almost exclusively 6.0 kb in size), followed by liver and lung ( 9.5 ≥ 6.0 kb iras mRNA ), kidney ( 6.0 > 9.5 kb ), heart (6.0 kb), spleen ( 6.0 ≥ 9.5 kb ), testes ( 6.0 > 9.5 kb ), and skeletal muscle ( 6.0 > 9.5 kb ). A correlation exists ( ρ= 0.71, p = 0.05 ) between total ( 6.0 + 9.5 kb ) iras mRNA and I 1 B MAX values across rat tissues, corrected for housekeeping gene expression. Thus, total iras mRNA appears to be roughly proportional to the density of I 1 ‐imidazoline binding sites.