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Stress Signal to Survival and Apoptosis a
Author(s) -
TAKEDA ATSUSHI,
TAKEDA NOBUAKIRA
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb09256.x
Subject(s) - myocarditis , medicine , dilated cardiomyopathy , apoptosis , myocyte , acute myocarditis , flow cytometry , cardiomyopathy , cardiology , inflammation , heart disease , pathology , endocrinology , heart failure , immunology , biology , biochemistry
A bstract : This investigation focused on whether apoptosis can be observed in some heart diseases. Apoptosis was examined immunochemically using monoclonal antibodies such as p53, Bcl‐2 and cyclin E, A, and B1 in parallel with flow cytometry. Left ventricular myocardium was obtained at autopsy from 40 patients with acute myocarditis (AM;N = 10 , 6 males, 4 females, mean age 56 ± 13 years), chronic myocarditis (CM;N = 10, 5 males, 5 females, mean age 48 ± 16 years), dilated cardiomyopathy (DCM;N = 10, 7 males, 3 females, mean age 60 ± 11 years), and no heart disease (Cont;N = 10, 5 males, 5 females, mean age 63 ± 14 years). Cell cycle analysis of myocytes by flow cytometry revealed that the relative content of G2M phase in acute myocarditis was far higher than those in other heart diseases (AM, 12.3 ± 3.7%; CM, 5.2 ± 4.5%; DCM, 6.3 ± 4.0%; Cont, 3.4 ± 1.8%; Mean ± SD). Expression of p53 was observed mainly in myocytes from chronic myocarditis. Expression of Bcl‐2, on the other hand, was detected in myocytes from acute myocarditis. Results suggest that apoptosis may play some role in the repairing process of myocardial inflammation.