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Cardiac Adaptation to Ischemia‐Reperfusion Injury a
Author(s) -
KINGMA JOHN G.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb09227.x
Subject(s) - ischemic preconditioning , cardioprotection , hsp70 , ischemia , cytoprotection , heat shock protein , reperfusion injury , shock (circulatory) , coronary occlusion , medicine , downregulation and upregulation , pharmacology , anesthesia , chemistry , oxidative stress , gene , biochemistry
A bstract : Acute myocardial ischemia initiates a cascade of cellular events that lead to irreversible injury. We previously described the transient nature of heat‐shock induced cardioprotection; treatment with a catalase inhibitor abolished the cytoprotective actions without affecting expression levels of HSP71. Repeated, transient ischemic episodes augment the ischemic tolerance of affected myocardium but the fundamental cytoprotective mechanism(s) for both “early” and “delayed” preconditioning remains unclear. Increased cellular induction of protooncogenes, heat shock genes, and downstream effector proteins might play critical roles in the cytoprotection afforded by delayed preconditioning. We measured c‐fos, c‐jun, c‐myc, and hsp70 induction in preconditioned (2 × 5‐min ischemia/10‐min reperfusion) and control rabbit hearts that either underwent 30‐ or 120‐min coronary occlusion and 60‐min reperfusion, or did not undergo subsequent sustained ischemia; the latter hearts were allowed to recover for 0, 1, 3, 6, 24, 48, 72, or 96 hours. Both c‐fos and c‐jun in ischemic tissue were strongly induced by ischemia‐reperfusion injury and preconditioning pretreatment. However, expression levels diminished significantly by 1‐h reperfusion and remained depressed during the 96‐h recovery period. Hsp70 (inducible) mRNA expression levels were highest primarily in ischemic myocardium after 6‐h recovery post‐preconditioning; Hsp70 levels in ischemic myocardium were slightly stronger after 48‐h recovery but subsequently diminished to barely detectable levels by 96‐h post‐preconditioning. Induction of c‐fos and c‐jun preceded that of Hsp70 . These findings support the concept that upregulation of immediate early genes and heat shock genes plays an important role in myocardial adaptation to acute ischemic stress.