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Amplification of Angiotensin II Signaling in Cardiac Myocytes by Adenovirus‐Mediated Overexpression of the AT 1 Receptor a
Author(s) -
BOOZ GEORGE W.,
CARL LOIS L.,
BAKER KENNETH M.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb09221.x
Subject(s) - angiotensin ii , myocyte , receptor , atrial myocytes , chemistry , medicine , angiotensin receptor , microbiology and biotechnology , endocrinology , biology
A bstract : Low levels of AT 1 receptor can make studying the growth‐related signal transduction events mediated by this angiotensin II receptor in cardiac myocytes technically difficult. The purpose of the present study was to establish whether an adenovirus expression system could be used to increase the number of plasma membrane AT 1 receptors in neonatal rat ventricular myocytes, thereby amplifying the signaling pathways activated by this receptor. Cardiac myocytes infected with adenovirus expressing the AT 1 receptor exhibited increased ligand binding. The overexpressed receptor appeared to function like the endogenous receptor, in regard to agonist‐induced internalization, as well as coupling to MAPK activation and protein tyrosine phosphorylation events. In addition, adenovirus‐mediated overexpression of the AT 1 receptor resulted in the amplification of angiotensin II intracellular signaling. In conclusion, adenovirus‐mediated overexpression of angiotensin II receptors appears to be a useful strategy for studying the signal transduction events activated by this hormone in cardiac myocytes and for unraveling the molecular means by which this receptor type couples to a hypertrophic pattern of growth and gene expression.