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Dissecting the Marrow Microenvironment
Author(s) -
TOROKSTORB BEVERLY,
IWATA MINEO,
GRAF LYNN,
GIANOTTI JOANN,
HORTON HEIDI,
BYRNE MICHAEL C.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb08461.x
Subject(s) - haematopoiesis , stromal cell , microbiology and biotechnology , progenitor cell , cell culture , biology , secretion , bone marrow , cd34 , function (biology) , cell fate determination , gene , stem cell , immunology , cancer research , genetics , transcription factor , biochemistry
A bstract : Cloned human stromal cell lines representing functionally distinct cellular components of the marrow microenvironment were generated to serve as tools for identifying gene products that regulate hematopoiesis. Oligonucleotide arrays, or “gene chips” were used to provide a comprehensive comparison of gene expression among the cell lines. One line, designated HS‐5, was found to secrete large amounts of cytokines, and conditioned media from this line was found to support the ex vivo expansion of both immature and mature progenitors. In contrast, a second line, designated HS‐27a, does not secrete known cytokines but does support cobblestone area formation by CD34 + /38 lo cells. HS‐27a, but not HS‐5, was also found to express hJagged1, a ligand for Notch1, which may function to influence cell fate decisions of hematopoietic precursors. Both cell lines are currently being used to identify other gene products that regulate hematopoiesis and to generate reagents that will allow more formal evaluation of the putative role of hJagged1 in hematopoietic cell fate decisions.