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Retroviral‐Fibronectin Interactions in Transduction of Mammalian Cells
Author(s) -
WILLIAMS DAVID A.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb08457.x
Subject(s) - transduction (biophysics) , fibronectin , microbiology and biotechnology , extracellular matrix , integrin , biology , stem cell , retrovirus , haematopoiesis , stromal cell , cell adhesion molecule , signal transduction , cell adhesion , cell , chemistry , cell culture , genetics , cancer research , biochemistry
A bstract : Hematopoiesis occurs in a complex environment in the medullary cavity in close proximity to stromal cells, fibroblasts, endothelial cells and matrix molecules. Hematopoietic cell interactions in this environment appear to involve both integrin and proteoglycan‐mediated cell‐cell and cell‐matrix interactions. Genetic transduction of hematopoietic stem cells via retroviral vectors has been hampered by low efficiency of gene transfer. Recently, hematopoietic stem cell adhesion to the extracellular matrix molecule fibronectin has been shown to increase transduction of these target cells using retrovirus vectors. The mechanism of increased transduction appears to involve colocalization of virus particles and target cells. These data are reviewed in this paper.