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Ultrastructural Alterations of Mitochondria in Pre‐apoptotic and Apoptotic Hepatocytes of TNFα‐treated Galactosamine‐sensitized Mice a
Author(s) -
ANGERMÜLLER S.,
SCHÜMANN J.,
FAHIMI H. D.,
TIEGS G.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb07918.x
Subject(s) - apoptosis , mitochondrion , microbiology and biotechnology , cytochrome c , cytochrome c oxidase , ultrastructure , chemistry , chromatin , prophase , inner membrane , biology , biochemistry , anatomy , dna , meiosis , gene
The electron microscopical studies presented here show that characteristic morphological alterations in mitochondria are a very early hallmark of the hepatocellular apoptotic program. Before chromatin condensation occurs, the outer mitochondrial membrane is focally disrupted and the inner membrane protrudes through this gap forming a hernia. The demonstration of cytochrome oxidase in mitochondria revealed a very strong activity in pre‐apoptotic and apoptotic cells as well as in apoptotic bodies.