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The Significance of Manduca sexta Allatostatin in the Tomato Moth Lacanobia oleracea
Author(s) -
AUDSLEY NEIL,
WEAVER ROBERT J.,
EDWARDS JOHN P.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb07903.x
Subject(s) - manduca sexta , corpus allatum , sphingidae , endocrinology , medicine , biology , juvenile hormone , noctuidae , neuropeptide , lepidoptera genitalia , biochemistry , larva , botany , hormone , receptor
A bstract : The nature and regulation of juvenile hormone (JH) biosynthesis have been investigated in isolated corpora allata (CA) of adult males and females, and larvae of the tomato moth Lacanobia oleracea (Noctuidae). Larval CA (V th day 1 to VIth day 1) appear to synthesize JH at very low rates (<0.5 fmol/pr/h). This synthesis was not affected by Manduca sexta allatostatin (Mas‐AS) nor Manduca sexta allatotropin. In contrast, adult female CA synthesize relatively high levels of JHI and JHII (>10 pmol/pr/h), each of which can be inhibited (approx. 60%) by Mas‐AS. CA from adult male L. oleracea do not produce detectable levels of JH but would appear to synthesize JH‐acids instead, which can also be inhibited (approx. 50%) by Mas‐AS. When assayed on adult female L. oleracea CA, brain extracts separated by liquid chromatography show inhibitory activity. The major biologically active fraction also has the greatest Mas‐AS‐like immunoreactivity and co‐elutes with synthetic Mas‐AS, indicating that most of the allatostatic activity in brain extracts is due to a Mas‐AS‐like peptide. In adult male brains, even though relatively high levels of immunoreactivity co‐elute with synthetic Mas‐AS, the majority of the Mas‐AS‐like immunoreactivity is more hydrophobic.