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Comparison of FaRP Immunoreactivity in Free‐living Nematodes and in the Plant‐parasitic Nematode Heterodera glycines
Author(s) -
MASLER E. P.,
KOVALEVA E. S.,
SARDANELLI S.
Publication year - 1999
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1999.tb07896.x
Subject(s) - heterodera , soybean cyst nematode , nematode , biology , fmrfamide , parasite hosting , zoology , neuropeptide , ecology , biochemistry , receptor , world wide web , computer science
A bstract : The family of FMRFamide‐related peptides (FaRPs) is widely distributed among invertebrates, where the peptides serve as neuromodulators. Published reports indicate that numerous FaRP sequences exist in free‐living and animal parasitic nematodes. Using a FMRFamide ELISA, FaRP immu‐noreactivity was detected in extracts of the soybean cyst nematode, Heterodera glycines , in both sexes and at all developmental stages. HPLC‐ELISA results revealed a number of immunoreactive components in H. glycines preparations, and a comparison with extracts of the free‐living nematodes Caenorhabditis el‐egans and Panagrellus redivivus showed significant qualitative differences in FaRP immunoreactivity between the plant parasite and the two free‐living nematodes. Total and specific immunoreactivities varied during H. glycines development, with the highest specific activity in juveniles and males, and the highest total activity in mature females. Total female immunoreactivity was located primarily within the mature eggs. A significant portion, however, was as‐sociated with the female body, perhaps with egg laying.