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High Expression of Inducible Nitric Oxide Synthase Correlates with Intestinal Inflammation of Interleukin‐2‐Deficient Mice
Author(s) -
HARREN M.,
SCHÖNFELDER G.,
PAUL M.,
HORAK I.,
RIECKEN E. O.,
WIEDENMANN B.,
JOHN M.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb11131.x
Subject(s) - nitric oxide synthase , inflammation , pathogenesis , colitis , western blot , messenger rna , immunology , nitric oxide , inflammatory bowel disease , interleukin , ulcerative colitis , immune system , biology , endocrinology , medicine , disease , cytokine , gene , biochemistry
Severely inflamed colonic sections of IL-2 (-/-) mice showed up to 19-fold increased iNOS mRNA levels. The level of iNOS protein expression corresponded to the increased iNOS mRNA levels as detected by means of Western blot analysis. There was a clear, positive relationship between the level of iNOS expression and the degree of inflammation in the colonic tissue of IL-2 (-/-) and wild-type mice. Our data suggest that iNOS may play a key role in the pathogenesis of ulcerative colitis-like disease in IL-2 (-/-) mice. Further investigation should elucidate the impact of NO on the regulation of the inflammatory process in this model and might contribute to a better understanding of the role iNOS expression in human immune-mediated diseases.