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Use of Tick Cell Culture‐derived Anaplasma marginale Antigen in a Competitive ELISA for Serodiagnosis of Anaplasmosis
Author(s) -
SALIKI JEREMIAH T.,
BLOUIN EDMOUR F.,
RODGERS SANDY J.,
KOCAN KATHERINE M.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb11059.x
Subject(s) - anaplasmosis , antigen , complement fixation test , antibody , virology , monoclonal antibody , biology , anaplasmataceae , microbiology and biotechnology , anaplasma , tick , immunology , serology
Anaplasma marginale was propagated in a continuous tick cell line and detergent‐solubilized infected cells were used as antigen in a competitive ELISA (C‐ELISA) for detection of Anaplasma‐specific antibody in bovine sera. Positive control sera competed well ( ≥35% inhibition) with an A. marginale ‐specific monoclonal antibody for binding to this antigen, while negative sera failed to compete ( <35% inhibition). The C‐ELISA was compared to the standard complement‐fixation test (CFT) using 2,208 bovine sera. Overall, C‐ELISA was more sensitive than CFT (24.9% versus 9.4%), mainly because CFT yielded “suspicious” or “anti‐complementary” results in 10.5% of the sera and also failed to identify several vaccinated and carrier cattle that were C‐ELISA‐positive. The apparent agreement between CFT and C‐ELISA was 89.6% and the kappa value was 0.6. These results show that this C‐ELISA would be a suitable replacement of the CFT as the standard test for detection of A. marginale antibody.