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A Partially Protective Clone from Cowdria ruminantium Identified by Using a Salmonella Vaccine Delivery System a
Author(s) -
BRAYTON K. A.,
WALT M.,
VOGEL S. W.,
ALLSOPP B. A.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb11055.x
Subject(s) - salmonella , clone (java method) , virology , biology , microbiology and biotechnology , recombinant dna , bacteria , gene , genetics
A strategy has been developed to screen the Cowdria genome, using a Salmonella vaccine delivery system, to identify genes that code for protection‐stimulating proteins. We have cloned mini‐libraries of Cowdria into this Salmonella system, used the recombinant bacteria to immunize outbred mice, and then challenged them after two weeks with a lethal dose of Cowdria . When one of these mini‐libraries was tested in a group of 5 mice, one mouse lived much longer than the others. The experiment was repeated with each of the clones from the mini‐library being tested individually in 10 mice, and one mouse survived the challenge. This clone has been tested repeatedly in larger groups of mice and is proven to protect 14% of outbred mice against a lethal Cowdria challenge.