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Cloning and Evaluation of the Role of Rat GALR‐2, a Novel Subtype of Galanin Receptor, in the Control of Pain Perception
Author(s) -
AHMAD SULTAN,
O'DONNELL DAJAN,
PAYZA KEM,
DUCHARME JULIE,
MÉNARD DANIEL,
BROWN WILLIAM,
SCHMIDT RALF,
WAHLESTEDT CLAES,
SHEN S. H.,
WALKER PHILIPPE
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10688.x
Subject(s) - galanin , galanin receptor , spinal cord , receptor , in situ hybridization , neuropeptide , open reading frame , chemistry , messenger rna , microbiology and biotechnology , amino acid , peptide , biology , peptide sequence , biochemistry , gene , neuroscience
A bstract : We have identified a novel subtype of galanin receptor (GALR‐2) in rat dorsal root ganglia and spinal cord. The open reading frame of GALR‐2 is 1116 nucleotides long, encoding a protein of 372 amino acids with a theoretical molecular mass of 40.7 kD. Membranes prepared from stable pools of 293 cells expressing GALR‐2, but not wild‐type 293 cells, demonstrated high affinity galanin binding sites. Rat galanin and galanin‐related peptides M40, C7, M15, and galanin 1–16 effectively competed for binding; peptide C7 demonstrated a lower affinity for rGALR‐2, and all these peptides were agonists at rGALR‐2 when assessed on a microphysiometer. Studies on the expression of GALR‐2 in various tissues by Northern and in situ hybridization analyses suggest a low abundance but wide distribution of GALR‐2 mRNA, including several discrete areas in brain and spinal cord and a high abundance in the dorsal root ganglia.