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Visualizing Odor Detection in Olfactory Cilia by Calcium Imaging a
Author(s) -
LEINDERSZUFALL TRESE,
GREER CHARLES A.,
SHEPHERD GORDON M.,
ZUFALL FRANK
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10567.x
Subject(s) - odor , cilium , cyclic nucleotide gated ion channel , calcium imaging , olfactory system , sensory system , neuroscience , olfactory epithelium , confocal , olfaction , chemistry , calcium , biophysics , biology , microbiology and biotechnology , physics , cyclic nucleotide , optics , biochemistry , nucleotide , organic chemistry , gene
To visualize odor detection in individual cilia of olfactory sensory neurons we have developed a new approach by using high‐resolution calcium imaging techniques. Laser scanning confocal microscopy revealed, for the first time, that odor stimuli induce transient Ca 2+ elevations in single olfactory cilia. Pharmacological analysis indicates that these Ca 2+ signals depend entirely on Ca 2+ entry through activated cyclic nucleotide‐gated (CNG) channels. This novel approach enables us to monitor the initial steps leading to olfactory perception in a spatially and temporally resolved manner.