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Multiple Intensified Performance of an Enzyme‐Catalyzed Reaction in Organic Medium
Author(s) -
YAMANE TSUNEO,
IWASAKI YUGO,
ROXANA ROSU,
SHIMIDZU NOBUYOSHI,
DOISAKI NOBUSHIGE
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10299.x
Subject(s) - lipase , chemistry , catalysis , transesterification , adsorption , polyunsaturated fatty acid , solvent , organic solvent , glycerol , organic chemistry , biocatalysis , fatty acid , enzyme , chemical engineering , reaction mechanism , engineering
A bstract : A lipase‐catalyzed glycerolysis reaction (a transesterification between polyunsaturated fatty acid ethyl ester [PUFA] and glycerol) was investigated. Its performance was multiply intensified by (1) using a lipase having high specific activity, high activity in organic solvent, and high tolerance in organic solvent; (2) immobilization on fine CaCO 3 powder (cheap and safe material, easy physical adsorption method of immobilization, reusable); (3) reaction in vacuo resulting in 100% conversion and effective avoidance of oxidative deterioration of PUFA; (4) high volumetric productivity because of no use of solvent; and (5) no need of further separation and purification of the oil product. It is emphasized that performance of biocatalytic reactions in organic media should be enhanced manifold for industrial implementation.