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Screening of Inhibitory Monoclonal Antibodies: A Critical Step for Producing Anti‐idiotypic Catalytic Antibodies
Author(s) -
AVALLE BÉRANGÈRE,
FRIBOULET ALAIN,
THOMAS DANIEL
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10294.x
Subject(s) - antibody , antigen , monoclonal antibody , epitope , complementarity (molecular biology) , chemistry , computational biology , microbiology and biotechnology , biology , immunology , genetics
A bstract : In accord with the original approach that we proposed, catalytic antibodies may be produced by using the anti‐idiotypic pathway according to antigen/antibody complementarity rules. The generation and screening of the idiotypic Ab1, the central point on which are anchored the interactions with both the antigen (enzyme) and the anti‐idiotypic abzyme, represent a crucial step for the success of this approach. We herein propose to describe a strategy for which we have developed a number of assays, aiming at selecting the proper Ab1, with desired features, likely to elicit an anti‐idiotypic catalytic antibody. β‐lactamase from Bacillus cereus was chosen as the example illustrating our arguments.