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Regulation of Levels of 5‐HT 2A Receptor mRNA
Author(s) -
WOHLPART K. L.,
MOLINOFF P. B.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10183.x
Subject(s) - receptor , downregulation and upregulation , protein kinase c , microbiology and biotechnology , gene isoform , heterologous , chemistry , signal transduction , messenger rna , 5 ht receptor , cell surface receptor , serotonin , biology , biochemistry , gene
ABSTRACT: We have been able to identify two separate pathways present in P11 cells that can increase the levels and stability of 5‐HT 2A receptor mRNA. One pathway is activated following exposure of cells to serotonin and is dependent upon activation of a PKC isoform that is susceptible to downregulation by a 24‐h pretreatment with PMA; another pathway is activated following treatment of cells with a calcium ionophore. This pathway is not dependent on activation of PKC isoforms that can be downregulated by a 24‐h treatment with phorbol ester. Such heterologous regulation of levels of 5‐HT 2A receptor mRNA may be important in understanding in vivo events where multiple factors contribute to the modulation of levels of cell surface receptors.