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Inducible Knockout Strategies to Probe the Functions of 5‐HT Receptors a
Author(s) -
STARK K. L.,
OOSTING R. S.,
HEN R.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10173.x
Subject(s) - gene knockout , knockout mouse , reporter gene , phenotype , gene , computational biology , biology , gene expression , gene targeting , microbiology and biotechnology , genetics
Gene targeting has proven to be extremely powerful in various fields of biological research. Through this technique, knockout mice lacking a particular gene and thus a particular protein, can be generated. One limitation to this technique is the fact that mice develop without the protein of interest and therefore, developmental compensations may have taken place, contributing to an observed phenotype. Inducible strategies, those which allow the timing of expression of a gene to be regulated, are currently being developed and should prove useful when applied to gene targeting technology. In order to begin to apply such new technologies to the field of gene targeting, we first created and tested several reporter constructions using the tetracycline inducible system. Here we describe the creation of several β‐galactosidase reporter constructions and the results of in vitro testing in Cos‐7 cells. We then discuss future knockout strategies based upon our observations.