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Measurement of Cell Volume Loss in the Liquid Region Preceding an Advancing Phase Change Interface a
Author(s) -
HARMISON HEATHER R.,
DILLER KENNETH R.,
WALSH JOHN R.,
NEILS CHRISTOPHER M.,
BRAND JERRY J.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb10162.x
Subject(s) - volume (thermodynamics) , redistribution (election) , phase (matter) , chemistry , front (military) , liquid phase , biophysics , thermodynamics , biology , meteorology , physics , organic chemistry , politics , political science , law
It is well understood that the solidification of a solution results in a redistribution of solute in the liquid zone. For the freezing of suspensions of cells it is anticipated that accumulation of solute in the region leading a growing ice phase will cause an osmotic response in cells before the ice phase reaches the cells. To measure this phenomenon in a specific algal species, the volume changes in Chlorococcum texanum during freezing were studied using directional solidification cryomicroscopy. The relative cell volume was tracked continuously as a function of temperature and position as cells encountered the moving phase front. The loss of cell volume was measured in the liquid region containing concentrated solute ahead of the growing solid phase.