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Significance of Gangliosides in Neuronal Differentiation of Neuroblastoma Cells and Neurite Growth in Tissue Culture
Author(s) -
RÖSNER HARALD
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb09672.x
Subject(s) - neurite , nerve growth factor , ganglioside , cell culture , chemistry , microbiology and biotechnology , neuroblastoma , cell growth , biology , biochemistry , in vitro , receptor , genetics
Addition of dl‐ threo ‐1‐phenyl‐2‐decanolylamine‐3‐morpholino‐1‐propanol HCl (PDMP; 7–24 μM) or Fumonisin B 1 (FB 1 ; 30–50 μM) to SH‐SY5Y trk‐A human neuroblastoma cells results within 4 days in a 40% decrease of the ganglioside content and in a reduction of nerve‐growth‐factor (NGF) ‐induced outgrowth of neuritic processes. NGF‐induced enhancement of GAP‐43 expression was not affected. However, unlike controls, immunostained GAP‐43 appeared concentrated in defined areas of cell perikarya and mostly absent from cell processes. Presence of 20‐μM exogenous GM1 for 4 days in NGF and PDMP containing cell cultures led to an increase of cell‐associated GM1 (15‐fold), GM2 (10‐fold), GM3 (15‐fold), GD1a (4‐fold), GD2, GD1b, and GT1b (all 3‐fold), and partially reversed the PDMP (and FB 1 ) effects on neurite growth and GAP‐43 distribution. In a newly developed neuronal tissue culture system, PDMP and FB 1 led to a comparable dose‐dependent inhibition of neurite outgrowth from embryonic chicken spinal cord explants, which had been embedded into a fibrin matrix. In this system, addition of GM1 led to a further inhibition of neurite growth, probably due to an interaction with growth‐promoting components present in the surrounding fibrin matrix.