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Metabolic Fate of Exogenous Sphingosine in Neuroblastoma Neuro2A Cells: Dose‐dependence and Biological Effects a
Author(s) -
RIBONI LAURA,
BASSI ROSARIA,
CAMINITI ANTONELLA,
PRINETTI ALESSANDRO,
VIANI PAOLA,
TETTAMANTI GUIDO
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb09661.x
Subject(s) - sphingosine , neuroblastoma , chemistry , dose dependence , biology , biochemistry , endocrinology , genetics , cell culture , receptor
The possible relationship between metabolism and biological effects of sphingosine was investigated in Neuro2a cells. [C3‐ 3 H]‐sphingosine, administered at different doses (80 pmol‐80 nmol/mg cell protein). Amounts up to hundredfold were rapidly taken up and metabolized, the intracellular content of sphingosine being processed within 2 h. At low doses, [ 3 H]‐sphingosine represented a minor portion of the cellular radiolabel, and N ‐acylated metabolites, particularly ceramide, prevailed over degradation products. Neuro2a cell differentiation took place in conjunction with ceramide increase. At increasing exogenous sphingosine/cell ratio, the acylation process became saturated while sphingosine degradation increased proportionally. From this point on [ 3 H]‐sphingosine accumulated and cell toxicity occurred. In conclusion, in Neuro2a cells the biological effects exerted by exogenous sphingosine are strictly connected to the exogenous sphingosine/cell ratio and to the capacity of the cell to metabolize sphingosine.