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Salivary Gland Nucleotide Receptors: Changes in Expression and Activity Related to Development and Tissue Damage a,
Author(s) -
TURNER JOHN T.,
PARK MINJUNG,
CAMDEN JEAN M.,
WEISMAN GARY A.
Publication year - 1998
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1998.tb09633.x
Subject(s) - receptor , nucleotide , salivary gland , biology , microbiology and biotechnology , endocrinology , medicine , genetics , gene , biochemistry
A bstract : Experiments were performed to document the presence of G protein‐coupled P2Y nucleotide receptors in rat salivary glands and to examine changes in receptor expression during development and under conditions in which gland architecture is altered. The results indicate that, as opposed to mature rat submandibular gland (SMG), immature glands express functional P2Y 1 receptors. P2Y 1 receptor activity was highest at birth and declined over the next four weeks to undetectable levels. P2Y 1 receptor mRNA levels remained constant over this time course, suggesting that receptor activity is regulated at some point other than transcription. Conversely, short‐term culture of cells from the three major salivary glands resulted in upregulation of functional P2Y 2 receptors. Responses to the P2Y 2 ‐selective agonist, UTP, were obtained after 3 h in culture and were maximal by 72 hours. This increase was paralleled by increased steady‐state P2Y 2 receptor mRNA levels. Upregulation of P2Y 2 receptors also occurred in vivo following ligation of the main excretory duct of the SMG. These studies suggest that nucleotide receptors are dynamically regulated during development and as a result of perturbations to gland architecture.