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Selected Aspects of the Microencapsulation of Mammalian Cells in HEMA‐MMA a
Author(s) -
SEFTON MICHAEL V.,
HWANG JEONG R.,
BABENSEE JULIA E.
Publication year - 1997
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1997.tb52201.x
Subject(s) - horseradish peroxidase , methacrylate , biocompatible material , capsule , membrane , confocal microscopy , chemistry , cell encapsulation , membrane permeability , viability assay , biophysics , cell , copolymer , materials science , biomedical engineering , biochemistry , microbiology and biotechnology , polymer , biology , organic chemistry , enzyme , medicine , botany
Microencapsulation of live mammalian cells is one means of creating hybrid artificial organs, like an artificial pancreas or an artificial liver. In addition to creating and developing the methodologies for enclosing cells within the appropriate semipermeable and biocompatible membranes, novel techniques are needed to assess the various features of the resulting capsules. The small size of a capsule or its heterogeneity can lead to additional complexities that go beyond the problem of examining cell behavior in the presence of biomaterials. These problems are illustrated here by comparison of protein release by microencapsulated HepG2 cells within large and small HEMA-MMA (hydroxyethyl methacrylate-methyl methacrylate) capsules, by assessment of the effect of processing conditions on HEMA-MMA microcapsule permeability to horseradish peroxidase at the individual capsule level, and by a confocal microscopy technique for assessing intracapsule cell viability.