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Influence of Interleukin‐1 Receptor Antagonist on [ 3 H] Serotonin and Histamine Release by Rat Basophilic Leukemia‐2H3 Cells
Author(s) -
CONTI PIO,
BARBACANE RENATO C.,
TRAKATELLIS MIRTO,
PLACIDO FERNANDA C.,
CATALDO IVANA,
REALE MARCELLA
Publication year - 1997
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1997.tb46250.x
Subject(s) - histamine , serotonin , endocrinology , medicine , receptor antagonist , 5 ht receptor , basophilic , secretagogue , receptor , antagonist , degranulation , chemistry , histamine h4 receptor , pharmacology , biology , histamine h2 receptor , pathology
Mast cells located in connective tissues are a potent source of vasoactive and inflammatory mediators, such as cytokines. They accumulate in tissues in a wide variety of diseases where their function in most cases in unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) (from 25.2 to 29.9%), and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-IRA-treated and -untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT spontaneous release from RBLC cultures.