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Triple‐Labeling Cytofluorimetric Quantitative and Qualitative Evaluation of Phagocytic Activity of Monocytes and Polymorphonuclear Cells
Author(s) -
MANCA E.,
ARANGINO V.,
LOMBARDINI S.,
GHIANI A.,
MUSU D.,
AMBROSINI B.,
GIACCO S. R.,
GIACCO G. S.
Publication year - 1997
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1997.tb46234.x
Subject(s) - medicine
Polymorphonuclear cells (PMNs) play an important role in the control of bacterial\udand mycotic infection and, partially, in the control of some viral infectious and some\udneoplasias. A nonsecondary role in the pathogenesis of several noninfectious diseases\udis also played. The study of PMN functions is needed to evaluate their activity\udin various immunodeficiencies. In other diseases, useful information about their activation state can also be learned.\udThe main limits to the study of PMN function so far have been: the need to isolate\udthe cells from peripheral blood (or other biological fluids); the need to use large\udblood volumes; the tests' complexity; the time-consuming nature of the tests; and the\uddifficulty in processing many samples at the same time. The traditional tests to evaluate\udphagocytosis are impaired by several technical limitations. A relevant improvement to routine tests for the evaluation of PMNs comes from\udmethods employing whole blood. Flow cytometry, because of its specific technical\udpeculiarities, provides the opportunity to study many different PMN functions in a\udnew and interesting way