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A Blocking ELISA for Detection of Antibody to a Subgroup‐Reactive Epitope of African Horsesickness Viral Protein 7 (VP7) Using a Novel Gamma‐Irradiated Antigen a
Author(s) -
HOUSE J. A.,
STOTT J. L.,
BLANCHARD M. T.,
LAROCCO M.,
LLEWELLYN M. E.
Publication year - 1996
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1996.tb53540.x
Subject(s) - epitope , monoclonal antibody , virology , antibody , african horse sickness , antigen , blocking antibody , conformational epitope , microbiology and biotechnology , chemistry , virus , biology , immunology
A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.