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Construction of Gene Expression System in Cultured Tobacco Cells a
Author(s) -
SHINMYO A.,
YOSHIDA K.,
KASAI T.
Publication year - 1996
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1996.tb40551.x
Subject(s) - enhancer , gene , gene expression , nicotiana tabacum , promoter , transcription (linguistics) , reporter gene , microbiology and biotechnology , biology , regulation of gene expression , horseradish peroxidase , chemistry , genetics , biochemistry , enzyme , linguistics , philosophy
To construct a gene expression system in cultured tobacco cells, useful regulatory elements of plant genes were studied. The promoter of the horseradish peroxidase gene, prxC2, showed high activity in tobacco cells, and it contained enhancer sequences and a cis element for wound induction. The heat shock promoter of the HSP18.2 gene from A. thaliana had strong activity of transcription when the incubation temperature of tobacco cells was shifted from 25 degrees C to 37 degrees C. These elements could be good candidates for foreign gene expression in tobacco cells.