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Identification of Novel Growth‐related Genes Linked to the Mitogenic Effect of PACAP on the Rat Pancreatic Acinar Cell Line, AR4‐2J
Author(s) -
SCHÄFER HEINER,
TRAUZOLD ANNA,
FÖLSCH ULRICH R.,
SCHMIDT WOLFGANG E.
Publication year - 1996
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1996.tb17554.x
Subject(s) - identification (biology) , gene , cell culture , line (geometry) , cell growth , biology , microbiology and biotechnology , acinar cell , endocrinology , medicine , pancreas , genetics , botany , geometry , mathematics
The mRNA differential display technique was employed in order to identify novel growth-related genes linked to the mitogenic effect of PACAP on AR4-2J cells. Hereby, three differentially expressed and PACAP-inducible genes were found, one of these being homologous to a recently discovered putative growth-related early response gene in mice. In AR4-2J cells, mRNA levels of this novel rat gene are increased by PACAP in a rapid and transient fashion. This effect was enhanced by cycloheximide, whereas actinomycin D prevented the stimulatory effect of PACAP.