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Cell Aggregation in a Chinese Hamster Ovary Cell Microcarrier Culture Affects the Expression Rate and N‐linked Glycosylation of Recombinant Mouse Placental Lactogen‐1 a
Author(s) -
BORYS MICHAEL C.,
LINZER DANIEL I. H.,
PAPOUTSAKIS ELEFTHERIOS T.
Publication year - 1994
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1994.tb44388.x
Subject(s) - chinese hamster ovary cell , placental lactogen , microcarrier , recombinant dna , glycosylation , human placental lactogen , cell culture , biology , extracellular , cricetulus , ovary , placenta , chemistry , microbiology and biotechnology , endocrinology , biochemistry , fetus , pregnancy , genetics , gene
A microcarrier culture of Chinese hamster ovary (CHO) cells, expressing the N-glycosylated recombinant protein mouse placental lactogen I (mPL-I), was found to form large cellular aggregates (400 to 600 microns in diameter). There was increased accumulation of lower molecular sized mPL-I glycoforms in cultures containing the large cellular aggregates at pH 7.3, but not at pH 7.6. Specific rates of mPL-I expression were found in the cultures with cellular aggregates at both pH values (7.3 and 7.6). These findings are interpreted in the light of our earlier studies that showed that extracellular pH and elevated ammonia concentrations affect both the glycosylation and the expression rates of mouse placental lactogen I.