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Use of Neurite Outgrowth as an in Vitro Method of Assessing Neurotoxicity
Author(s) -
ABDULLA ELIZABETH McFARLANE,
CAMPBELL IAIN C.
Publication year - 1993
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1993.tb18308.x
Subject(s) - neurite , neurofilament , neurotoxicity , amyotrophic lateral sclerosis , glutamate receptor , kainate receptor , in vitro , neurodegeneration , neuroscience , excitotoxicity , chemistry , microbiology and biotechnology , biochemistry , biology , pharmacology , immunology , toxicity , medicine , pathology , receptor , disease , immunohistochemistry , organic chemistry , ampa receptor
This work shows that the neurotoxic excitatory amino acids beta-N-methylamino alanine, BMAA, and kainate, modulate neurite outgrowth; this was assessed by measuring the levels of two separate neurofilament proteins (68 kD and 160 kD), in a mouse neuroblastoma cell line, (NB41A3). BMAA has been proposed to be the exogenous excitotoxin in Guam disease or amyotrophic lateral sclerosis (ALS/parkinsonian/dementia; Guam ALS-PD). Kainate is a glutamate analogue which causes excitotoxic damage associated with excessive entry of calcium into neurons. The results show that at low doses (10(-9) to 10(-7) M) both BMAA and kainate decrease the concentration of the two neurofilament proteins. However at high doses (10(-6) to 10(-5) M) they cause an apparent accumulation of the neurofilament proteins; the effect is more marked with BMAA. These results support the continued development of an in vitro test for neurotoxicity based on neurite outgrowth.
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