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Growth Suppression of Normal Mammary Epithelial Cells by Wild‐Type p53
Author(s) -
MERLO GIORGIO R.,
VENESIO TIZIANA,
TAVERNA DANIELA,
CALLAHAN ROBERT,
HYNES NANCY E.
Publication year - 1993
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1993.tb17195.x
Subject(s) - wild type , allele , mutation , biology , gene , splice , microbiology and biotechnology , cancer research , genetics , mutant
We examined the status of the p53 gene in the HC11 normal mammary epithelial cells. Two mutations were identified: a Cys to Trp change at codon 138 and a microdeletion of codon 123 to 130 resulting from mutation of the splice acceptor site. These two mutations were independent, and no wild-type p53 allele was found. Introduction of wt-p53 strongly inhibited growth in monolayer. Thus, the absence of wt-p53 can be sufficient for the immortalization of mammary cells.