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Identification and Partial Characterization of the Salmon Calcitonin/CGRP Gene by Polymerase Chain Reaction
Author(s) -
JANSZ H. S.,
ZANDBERG J.
Publication year - 1992
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1992.tb22757.x
Subject(s) - polymerase chain reaction , calcitonin gene related peptide , identification (biology) , gene , chemistry , polymerase , calcitonin , computational biology , biology , biochemistry , endocrinology , neuropeptide , receptor , botany
The polymerase chain reaction was used to amplify sequences encoding calcitonin and CGRP in the genomic DNA of salmon. Amplification products of the expected length were cloned and sequenced. In this way a new CGRP-coding sequence was identified. The new sequence and the known salmon calcitonin-coding sequence were shown to be part of one gene, implying that alternative gene expression takes place in fish.